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pe anti mouse cd11c  (Proteintech)


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    Structured Review

    Proteintech pe anti mouse cd11c
    Pe Anti Mouse Cd11c, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd11c/PE+Anti-Mouse+CD11c/pm41362070-184-11-16
    Average 93 stars, based on 10 article reviews
    pe anti mouse cd11c - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Diradical‐Featured Organic Small‐Molecule Photothermal Material Based on 4,6‐di(2‐thienyl)thieno[3,4‐c][1,2,5]thiadiazole for Photothermal Immunotherapy
    Article Snippet: Photothermal immunotherapy can provide an efficient and convenient strategy for cancer treatment by inducing immunogenic cell death (ICD) to generate vaccine-like functions in situ, which highly relies on suitable photothermal agents with superior photothermal effect.. Herein, a novel diradical-featured organic small-molecule photothermal material (TTD-TPE) based on 4,6-di(2-thienyl)thieno[3,4-c][1,2,5]thiadiazole and tetraphenylethylene is developed to exhibit near-infrared (NIR) absorption of 808 nm, high photothermal conversion efficiency (PTCE) of about 75%, and superior photoacoustic imaging (PAI) property in nanoparticle.. The excellent photothermal effect of TTD-TPE nanoparticles originates from the integrated synergic effect of D–A interactions, diradical character, and excited-state intramolecular motion, which not only ablates the primary tumor by localized high temperature but also transforms the primary tumor into vaccine in situ through ICD to inhibit the growth of distant and metastatic tumors, demonstrating the huge potential in photothermal immunotherapy.

    Article Title: High-Yield Outer Membrane Vesicles Derived From Probiotics as a Nanoplatform for Precise Treatment and Prophylaxis of Pseudomonas aeruginosa Infection.
    Article Snippet: .. The BMDCswere washed with PBS for three times and incubated with PE anti-mouse CD11c (cat# PE-65130, Proteintech, USA) in the dark at 4◦C for 1 h. After washing with PBS for three times, the uptake level of BMDCs was detected by flow cytometry (BD Accuri C6, BD Biosciences, USA). ..

    Binding Assay:

    Article Title: Diradical‐Featured Organic Small‐Molecule Photothermal Material Based on 4,6‐di(2‐thienyl)thieno[3,4‐c][1,2,5]thiadiazole for Photothermal Immunotherapy
    Article Snippet: Photothermal immunotherapy can provide an efficient and convenient strategy for cancer treatment by inducing immunogenic cell death (ICD) to generate vaccine-like functions in situ, which highly relies on suitable photothermal agents with superior photothermal effect.. Herein, a novel diradical-featured organic small-molecule photothermal material (TTD-TPE) based on 4,6-di(2-thienyl)thieno[3,4-c][1,2,5]thiadiazole and tetraphenylethylene is developed to exhibit near-infrared (NIR) absorption of 808 nm, high photothermal conversion efficiency (PTCE) of about 75%, and superior photoacoustic imaging (PAI) property in nanoparticle.. The excellent photothermal effect of TTD-TPE nanoparticles originates from the integrated synergic effect of D–A interactions, diradical character, and excited-state intramolecular motion, which not only ablates the primary tumor by localized high temperature but also transforms the primary tumor into vaccine in situ through ICD to inhibit the growth of distant and metastatic tumors, demonstrating the huge potential in photothermal immunotherapy.

    Staining:

    Article Title: Diradical‐Featured Organic Small‐Molecule Photothermal Material Based on 4,6‐di(2‐thienyl)thieno[3,4‐c][1,2,5]thiadiazole for Photothermal Immunotherapy
    Article Snippet: Photothermal immunotherapy can provide an efficient and convenient strategy for cancer treatment by inducing immunogenic cell death (ICD) to generate vaccine-like functions in situ, which highly relies on suitable photothermal agents with superior photothermal effect.. Herein, a novel diradical-featured organic small-molecule photothermal material (TTD-TPE) based on 4,6-di(2-thienyl)thieno[3,4-c][1,2,5]thiadiazole and tetraphenylethylene is developed to exhibit near-infrared (NIR) absorption of 808 nm, high photothermal conversion efficiency (PTCE) of about 75%, and superior photoacoustic imaging (PAI) property in nanoparticle.. The excellent photothermal effect of TTD-TPE nanoparticles originates from the integrated synergic effect of D–A interactions, diradical character, and excited-state intramolecular motion, which not only ablates the primary tumor by localized high temperature but also transforms the primary tumor into vaccine in situ through ICD to inhibit the growth of distant and metastatic tumors, demonstrating the huge potential in photothermal immunotherapy.

    Article Title: Self-assembly of paclitaxel derivative and fructose as a potent inducer of immunogenic cell death to enhance cancer immunotherapy
    Article Snippet: .. The co-culture systems were treated with NS, PTX injections (2 μg/mL), PTX-PBA-Fru NPs (2.35 μg/mL, equivalent to 2 μg/mL of PTX), R848 solution (0.5 μg/mL, dissolved in NS) and a combination of PTX-PBA-Fru NPs and R848-Lipo (2.35 μg/mL of PTX-PBA and 0.5 μg/mL of R848) for 12 h. After treatment, the cells were collected and stained with the following antibodies: CD45 monoclonal antibody (PerCP-Cyanine5.5, eBioscience), PE anti-mouse CD11c, FITC anti-mouse CD80, and APC anti-mouse CD86 (Proteintech). ..

    Flow Cytometry:

    Article Title: High-Yield Outer Membrane Vesicles Derived From Probiotics as a Nanoplatform for Precise Treatment and Prophylaxis of Pseudomonas aeruginosa Infection.
    Article Snippet: .. The BMDCswere washed with PBS for three times and incubated with PE anti-mouse CD11c (cat# PE-65130, Proteintech, USA) in the dark at 4◦C for 1 h. After washing with PBS for three times, the uptake level of BMDCs was detected by flow cytometry (BD Accuri C6, BD Biosciences, USA). ..



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    Proteintech anti mouse cd11c
    In vitro immune response of antigen‐presenting cells activated by FI‐mOMVs. (a) Confocal microscopy images of antigen uptake by BMDCs after incubation with the indicated formulations for 6 h. The cell immunofluorescence staining was performed by the marker protein <t>CD11c</t> on the cell membrane. The cell nuclei were stained with DAPI. Scale bar, 100 µm. (b, c) Flow cytometry was used to detect the uptake efficiency of FI antigen after incubation with BMDCs (b), and the uptake level was quantified (c) ( n = 3). (d) The uptake efficiency of BMDCs on unbroken FI‐mOMVs and broken FI‐mOMVs was detected by flow cytometry ( n = 3). (e, f) Flow cytometry was performed to measure the percentage of CD80 + (e) or CD86 + (f) cells in CD11c + BMDCs after incubation with the indicated formulations for 24 h ( n = 3). (g) FITC‐FI, DiI‐mOMVs and DiI‐FI‐mOMVs were incubated with PMs for 6 h. The cell immunofluorescence staining was performed by the marker protein F4/80 on the cell membrane. After DAPI staining, laser scanning confocal microscopy was used to detect the uptake of FI‐mOMVs by PMs. Scale bar, 100 µm. (h, i) Flow cytometry was used to quantitatively detect the uptake level of FI antigen by PMs (h), and quantitative analysis was performed (i) ( n = 3). (j) The activities of immune‐related enzymes ACP, LDH, iNOS in PMs were detected by ELISA after different treatments for 12 h ( n = 3). (k) Production of TNF‐α, IL‐6, TGF‐β and IL‐10 in the cell supernatant measured by ELISA after different treatments for 12 h ( n = 3). The data are presented as mean ± SEM. Statistical analysis was performed by a two‐tailed unpaired Student's t ‐test, and differences were considered significant at p < 0.05. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001 and **** p < 0.0001.
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    Image Search Results


    In vitro immune response of antigen‐presenting cells activated by FI‐mOMVs. (a) Confocal microscopy images of antigen uptake by BMDCs after incubation with the indicated formulations for 6 h. The cell immunofluorescence staining was performed by the marker protein CD11c on the cell membrane. The cell nuclei were stained with DAPI. Scale bar, 100 µm. (b, c) Flow cytometry was used to detect the uptake efficiency of FI antigen after incubation with BMDCs (b), and the uptake level was quantified (c) ( n = 3). (d) The uptake efficiency of BMDCs on unbroken FI‐mOMVs and broken FI‐mOMVs was detected by flow cytometry ( n = 3). (e, f) Flow cytometry was performed to measure the percentage of CD80 + (e) or CD86 + (f) cells in CD11c + BMDCs after incubation with the indicated formulations for 24 h ( n = 3). (g) FITC‐FI, DiI‐mOMVs and DiI‐FI‐mOMVs were incubated with PMs for 6 h. The cell immunofluorescence staining was performed by the marker protein F4/80 on the cell membrane. After DAPI staining, laser scanning confocal microscopy was used to detect the uptake of FI‐mOMVs by PMs. Scale bar, 100 µm. (h, i) Flow cytometry was used to quantitatively detect the uptake level of FI antigen by PMs (h), and quantitative analysis was performed (i) ( n = 3). (j) The activities of immune‐related enzymes ACP, LDH, iNOS in PMs were detected by ELISA after different treatments for 12 h ( n = 3). (k) Production of TNF‐α, IL‐6, TGF‐β and IL‐10 in the cell supernatant measured by ELISA after different treatments for 12 h ( n = 3). The data are presented as mean ± SEM. Statistical analysis was performed by a two‐tailed unpaired Student's t ‐test, and differences were considered significant at p < 0.05. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001 and **** p < 0.0001.

    Journal: Journal of Extracellular Vesicles

    Article Title: High‐Yield Outer Membrane Vesicles Derived From Probiotics as a Nanoplatform for Precise Treatment and Prophylaxis of Pseudomonas aeruginosa Infection

    doi: 10.1002/jev2.70194

    Figure Lengend Snippet: In vitro immune response of antigen‐presenting cells activated by FI‐mOMVs. (a) Confocal microscopy images of antigen uptake by BMDCs after incubation with the indicated formulations for 6 h. The cell immunofluorescence staining was performed by the marker protein CD11c on the cell membrane. The cell nuclei were stained with DAPI. Scale bar, 100 µm. (b, c) Flow cytometry was used to detect the uptake efficiency of FI antigen after incubation with BMDCs (b), and the uptake level was quantified (c) ( n = 3). (d) The uptake efficiency of BMDCs on unbroken FI‐mOMVs and broken FI‐mOMVs was detected by flow cytometry ( n = 3). (e, f) Flow cytometry was performed to measure the percentage of CD80 + (e) or CD86 + (f) cells in CD11c + BMDCs after incubation with the indicated formulations for 24 h ( n = 3). (g) FITC‐FI, DiI‐mOMVs and DiI‐FI‐mOMVs were incubated with PMs for 6 h. The cell immunofluorescence staining was performed by the marker protein F4/80 on the cell membrane. After DAPI staining, laser scanning confocal microscopy was used to detect the uptake of FI‐mOMVs by PMs. Scale bar, 100 µm. (h, i) Flow cytometry was used to quantitatively detect the uptake level of FI antigen by PMs (h), and quantitative analysis was performed (i) ( n = 3). (j) The activities of immune‐related enzymes ACP, LDH, iNOS in PMs were detected by ELISA after different treatments for 12 h ( n = 3). (k) Production of TNF‐α, IL‐6, TGF‐β and IL‐10 in the cell supernatant measured by ELISA after different treatments for 12 h ( n = 3). The data are presented as mean ± SEM. Statistical analysis was performed by a two‐tailed unpaired Student's t ‐test, and differences were considered significant at p < 0.05. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001 and **** p < 0.0001.

    Article Snippet: The BMDCs were collected and then labelled by PE anti‐mouse CD11c (cat# PE‐65130, Proteintech, USA), FITC anti‐mouse CD80 (cat# FITC‐65076, Proteintech, USA) and FITC anti‐mouse CD86 (cat# FITC‐65068, Proteintech, USA).

    Techniques: In Vitro, Confocal Microscopy, Incubation, Immunofluorescence, Staining, Marker, Membrane, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Two Tailed Test